Original Research ● Open access
doi https://doi.org/10.56003/pessr.v3i1.346

Correlation Between Blood Lactate and Salivary Biomarkers During Exercise in Women with Hypothyroidism Treated With L-Thyroxine

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Authors

  • Romeu Paulo Martins Silva , 
  • Alexandre Gonçalves , 
  • Carolina Freitas da Silva , 
  • Miguel Júnior Sordi Bortolini , 
  • Elmiro Santos Resende , 
  • Luis Carlos Oliveira Gonçalves , 
  • Aníbal Monteiro de Magalhães-Neto , 
  • Foued Salmen Espindola

Abstract

Background: Hypothyroidism reduces circulating levels of thyroid hormones metabolic enzymatic activities, and the skeletal muscle's work capacity and lactatemia increase during exertion.

Objectives: To analyze salivary biomarkers and blood lactate obtained in exercise stages through exhaustion and investigate their correlation.

Methods: Eight females attended the University Clinical Hospital who showed a condition of subclinical hypothyroidism. Before they began replacing with L-thyroxine, they were submitted to an ergometric test to check their tolerance to effort. Eight weeks after treatment, all volunteers were again submitted to lab and ergometric tests. Saliva and blood were collected to determine total protein, nitric oxide, IgA, lactate, and lipid profile.

Results: The present study identified a correlation (0.8), so the behavior of the blood lactate could explain the behavior of the salivary of total proteins by 74.22% (R2 = 0.7422). Lactate production was minimized when women underwent L-thyroxine treatment in the first six stages of exercise. Still, total salivary proteins increased for pharmacotherapy between stages 1 and 8. While salivary nitric oxide had a percentage variation between pre- and post-exercise times of 96% before the start of treatment when the same exercise was performed after eight weeks of treatment with L-Thyroxine, this variation was 150%.

Conclusion: The measurement of STP is an essential marker of stress, and it is highly correlated with the most used blood marker, lactate. Regarding treatment with L-Thyroxine in women with subclinical hypothyroidism, after eight weeks of this therapy, it was possible to observe a reduction in blood lactate production after the incremental load test on a cycle ergometer, accompanied by a reduction in the concentration of SA. SNO and SIgA tended to increase in women undergoing treatment, and this treatment should be considered in subsequent studies as causing a different effect on these markers.

Keywords:

Exercise immunometabolism sportomics public health

Author Biographies

  • Romeu Paulo Martins Silva, Federal University of Catalão, Brazil

    He holds a degree in Physical Education from Centro Universitário do Triângulo (2003). Specialist in Exercise Physiology from the Veiga de Almeida University, UVA/RJ (2003/2005). Master in Genetics and Biochemistry from the Federal University of Uberlândia (2007). PhD in Genetics and Biochemistry (2011). Professor of the subjects of Human Anatomy, Embryology and Pathology at the Federal University of Catalão, collaborating professor in the Postgraduate Programs in Health Sciences in the Western Amazon and permanent in the master's degree in Sciences, Innovation and Technology for the Amazon (Ufac), permanent professor at Postgraduate Program in Nutrition and Health at the Federal University of Espirito Santos. He has experience in the areas of Human Anatomy, Biochemistry, Human Physiology, Exercise Physiology. Working mainly on the following topics: natural products to control non-communicable diseases (diabetes, heart disease and cancer), improve health conditions and physical performance; steroids and anabolic steroids.

  • Miguel Júnior Sordi Bortolini, Federal University of Acre, Rio Branco - AC, 69920-900, Brazil

    Exclusive Dedication Professor, Federal University of Acre (Ufac); e-mail: MIGUEL.BORTOLINI@UFAC.BR / BORTOLINIMJS@GMAIL.COM Coordinator (06/2019 to 10/2021) and Advisor (2016 to Present) of the Postgraduate Program in Health Sciences in the Western Amazon (PPGCSAO) ; Disciplines of Immunology (Medicine, Physical Education, Nutrition), Physical Activity and Health (PPGCSAO), Integrated Health Practices (Physical Education, Nursing, Medicine, Nutrition, Psychology and Public Health); Physical Activity in Health Promotion (Medicine); Research Methodology (Lic. Ed. Physics); Basketball (Bachelor and Degree in Physical Education); PET Tutor - Physical Education (2019/July - Present) PET Coordinator GraduaSUS/Physical Education (2016 to 2018); Scientific Initiation Advisor; Coordinator of the Translational Immunology Laboratory (LABIT); Coordinator of LAMED - Acriana Academic League of Sports Medicine, Nutrition and Exercise Immunology; Columnist at Rádio CBN (2018-2020 - Central Brasileira de Notícias/Acre) for the Saúde em Movimento Column; Journal reviewer (International Journal of Sports Medicine, Archives of Endocrinology and Metabolism, ...); He worked in Public Health as a Physical Trainer (Lectures and Practical Physical Activity Groups) at the Integrated Care Unit (UAI/UPA) - São Jorge - Uberlândia, Minas Gerais; Youtube Channel: Demystifying Immunology; PodCast on Spotify - ImmunEx&Health

  • Luis Carlos Oliveira Gonçalves, Federal University of Mato Grosso - MT, 78060-900, Brazil

    Graduated in Physical Education (Degree and Bachelor); Graduated in Pharmacy (Bachelor’s degree); Graduating in statistics (Bachelor’s degree); 04 specializations; Master in Human Motricity Science (Biochemistry); PhD in Health Sciences from the UFMT Faculty of Medicine (Physiology and Biochemistry); Professor of Biochemistry, Metabolism, Cell Biology, Immunology, Pharmacology, Physiology and Biomechanics (UNIMSB/UNIRJ/UNIESP) 2007-2021; Effective Pharmacist at the City of Itaguaí, Effective Pharmacist at the City of Angra dos Reis. Member of the Protein Biochemistry Laboratory - UNIRIO from 2005 to 2022; Member of the Laboratory of Biochemistry and Molecular Biology (LABIBI) at the Federal University of Uberlândia since 2024 and of the Research Group in Physiology and Metabolism - UFMT since 2019. Reviewer of the magazines Panorâmica - UFMT, South American Journal of Basic Education, Technical and Technological, Journal of Sports Sciences, Journal of Pharmaceutical Research, African Educational Research Journal, MEDPRESS Public Health and Epidemiology, Asian Journal of Research in Medical and Pharmaceutical Sciences, Biomedical Sciences, International Journal of Research and Reports in Dentistry, Scientific Reports; President of the Pharmacy and Therapeutics Commission of the Municipality of Itaguaí in the 2016/2017 biennia; 2018/2019; 2020/2021 and 2022/2023, General Coordinator of Pharmaceutical Assistance for Itaguaí in 2016, coordinator of the health demands program 2016/2017 and General Coordinator of Health Surveillance for the Municipality of Itaguaí/RJ 2020-2022. Post-Doctorate from the Postgraduate Program in Basic and Applied Immunology and Parasitology (PPGIP) UFMT-Araguaia 2022-2023; Post-Doctorate from the Postgraduate Program in Genetics and Biochemistry (UFU) 2024-2025; Collaborating professor of the master's degree (PPGEF) UFMT-Cuiabá; MBA in progress in Data Science and Analytics (USP)

  • Aníbal Monteiro de Magalhães-Neto, Federal University of Mato Grosso - MT, 78060-900, Brazil

    He has a degree in Physical Education from the School of Physical Education of Goiás (1999), a master's degree in Physical Education from the Catholic University of Brasília (2002), a Master's degree in Genetics and Biochemistry from the Federal University of Uberlândia (2007). PhD in Genetics and Biochemistry from the Federal University of Uberlândia (2011). Associate Professor at the Federal University of Mato Grosso. I have experience in the area of Physical Education, with an emphasis on Physical Activity, working mainly on the following topics: Salivary Biomarkers, Diabetes, Obesity and Physical Exercise. I worked as a member of Team Brazil during the Summer Olympic Games in the city of Rio de Janeiro in August 2016. In the project Changes in the metabolome of high-performance athletes: a tool for understanding hypermetabolic states, being supervised by L.C.Cameron. And also in school Physical Education: With adventure body practices (orienteering sport) and their nuances in the cognitive abilities of students at different stages of basic, elementary and secondary education. I am the coordinating member of the Research Group (Borders, Cultures, Identities: space for dialogue with indigenous peoples of Araguaia/Xingu (Focus on interaction between Indigenous people and sport) inside and outside the School (Address to access this mirror: dgp.cnpq.br /dgp/espelhogrupo/8469261688263537). Life has taught me that regardless of the laboratory (clinical analyses, the mass spectrometry room until we get to the classroom, or a multi-sports court inside or outside the school. YES IT IS POSSIBLE, to obtain WONDERFUL RESULTS

  • Foued Salmen Espindola, Federal University of Uberlandia - MG, 38405-320, Brazil

    Full Professor of Biochemistry at the Federal University of Uberlândia (UFU). He holds a bachelor's degree in Biological Sciences (1978) and Pharmacy/Biochemistry (1980), with a master's degree in Biochemistry and Immunology (1983), all from the Federal University of Minas Gerais (UFMG). PhD in Sciences (Biochemistry) from the University of São Paulo (USP) and post-doctorate in Cellular Biology and Biochemistry from Yale University (1994-1997; 1999-2000), as a fellow of the Pew Latin American Fellows Program in Biomedical Sciences (USA) and CNPq (Brazil). Teacher and advisor in the following postgraduate programs at UFU: Health Sciences (PPCSA), Genetics and Biochemistry (PPGGB) and Applied Cellular and Structural Biology (PPGBC). The research areas are biochemistry, molecular and cellular biology, biotechnology and nanotechnology, with an emphasis on oxidative stress, inflammation, diabetes, natural products, physical exercise and diagnosis of salivary biomarkers. He also develops research, innovation and extension on oxidative stress in health and disease and on medicinal plants and natural products from the biodiversity of the Cerrado biome, for therapeutic target studies. http://www.researcherid.com/rid/C-5324-2013

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Vol. 3 No. 1 (2024): Physical Education and Sports: Studies and Research
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Original Research
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05 May 2024
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https://doi.org/10.56003/pessr.v3i1.346

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Copyright (c) 2024 Romeu Paulo Martins Silva, Alexandre Gonçalves, Carolina Freitas da Silva, Miguel Júnior Sordi Bortolini, Elmiro Santos Resende, Luis Carlos Oliveira Gonçalves, Aníbal Monteiro de Magalhães-Neto, Foued Salmen Espindola

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Silva, R. P. M. , Gonçalves, A. , Silva, C. F. D. , Bortolini, M. J. S. , Resende, E. S. , Gonçalves, L. C. O. , Magalhães-Neto, A. M. D. , & Espindola, F. S. (2024). Correlation Between Blood Lactate and Salivary Biomarkers During Exercise in Women with Hypothyroidism Treated With L-Thyroxine. Physical Education and Sports: Studies and Research, 3(1), 57-76. https://doi.org/10.56003/pessr.v3i1.346

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How to Cite

Correlation Between Blood Lactate and Salivary Biomarkers During Exercise in Women with Hypothyroidism Treated With L-Thyroxine. (2024). Physical Education and Sports: Studies and Research, 3(1), 57-76. https://doi.org/10.56003/pessr.v3i1.346